Catalog Number - 59150-04

MicroBee Amino™ is a traditional aminopropyl HPLC stationary phase developed for laboratories requiring a proven alternative to legacy amino column methods. As part of the Cogent™ Similars product line, it has been widely used for many years in carbohydrate analysis, normal phase chromatography, and other applications where amino selectivity is desired. The stationary phase consists of a bonded amino functional group attached to high-purity Type-A silica, providing retention characteristics comparable to traditional amino columns used in established methods.

59150-04 is a 150 mm x 3.9 mm ID analytical column packed with 10 µm, 125Å MicroBee Amino particles. This dimension closely matches the format used in many historical HPLC methods developed on Microbondapak™ Amino and similar columns, making it particularly useful for method transfer and replacement applications. The 150 mm length provides a balance between resolution and run time, while the 3.9 mm ID format typically performs well at flow rates of approximately 0.8–1.5 mL/min on conventional HPLC systems.

This USP L8 column is an excellent alternative for methods specifying Microbondapak™ Amino and similar stationary phases. Packed and shipped in normal phase solvents, it is optimized for normal phase chromatography and related separations. The aminopropyl functionality provides selectivity useful for carbohydrate analysis, sugar profiling, geometric isomer separations, and other applications where traditional amino phases continue to be preferred.

Key Benefits

• Traditional aminopropyl stationary phase

• Suitable alternative for USP L8 methods

• Compatible with many legacy Microbondapak™ methods

• Optimized for normal phase chromatography

• 125Å pore size for broad analyte compatibility

• Cost-effective Cogent Similars column

• Suitable for carbohydrate and sugar analysis

• Ideal for method transfer applications

Typical Applications

• USP L8 methods

• Normal phase HPLC

• Carbohydrate analysis

• Sugar separations

• Food and beverage testing

• Pharmaceutical analysis

• Method transfer and validation

• Geometric isomer separations

Specifications

• Product Type: HPLC Column

• Stationary Phase: MicroBee Amino

• USP Classification: L8

• Particle Size: 10 µm

• Particle Type: Type-A Silica

• Bonded Phase: Aminopropyl

• Endcapped: No

• Pore Size: 125Å

• Column Dimensions: 3.9 mm ID x 150 mm

• Length: 150 mm

• Recommended Flow Rate: 0.8–1.5 mL/min

• Shipping Solvent: Normal Phase

• Brand: Cogent

Important Notes

• Packed and shipped in normal phase solvents

• Designed primarily for normal phase chromatography

• USP L8 column classification

• Ideal for legacy method replacement

• Compatible with conventional HPLC systems

• Suitable for carbohydrate and sugar analysis

• Comparable to traditional Microbondapak™ Amino columns

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